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R26-CAG-LSL-RSR-tdTomato-2A-DTR

原創發布者：北檢院發布時間：2023-03-16 點擊數：

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品系介紹

品系全名：C57BL/6JSmoc-Gt(ROSA)26Sor^{em1(CAG-LSL-RSR-tdTomato-2A-DTR)Smoc}

目錄號：NM-KI-190086

品系狀態：活體

將CAG-LSL-RSR-tdTomato-2A-DTR插入到小鼠Rosa26基因中。

基本信息

基因名：Gt(ROSA)26Sor

基因曾用名：R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID：14910

MGI ID：104735

Ensembl ID：ENSMUSG00000086429

Pubmed：Gt(ROSA)26Sor

相關數據

Fig1. Immunostaining for tdTomato on sections of multiple tissues or organs collected from ACTB-Cre;R26-LR-tdT-DTR mice (upper panel) or CAG-Dre;R26-LR-tdT-DTR mice (lower panel).

Fig2. DTR-mediated cardiomyocyte ablation after DT administration.

A, a schematic figure showing experimental design. B, whole-mount view of triple transgenic mice treated with PBS or DT. Yellow scale bar represents 2000 μm. C, immunostaining for DTR and tdTomato on heart sections of triple transgenic mice. White scale bar represents100 μm. D, immunostaining for cTnT and tdTomato on heart sections of triple transgenic mice. White scale bar represents 100 μm. E, immunostaining for Tunel and tdTomato on heart sections. White scale bar represents 100 μm.

Fig3. DTR-mediated specific ablation of Cyp2e1⁺ peri-central hepatocytes.

A, a cartoon figure showing the mouse’s mating strategy. B, whole-mount epifluorescence view of livers collected from mice treated with DT or PBS. Yellow scale bar represents 2000 μm. C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections treated with PBS. White scale bar represents 100 μm. C, central vein. E, immunostaining for CYP2E1, tdTomato, and E-cad on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm. F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison of tdTomato+ hepatocytes (PBS group). G, immunostaining for Tunel and tdTomato on liver sections from mice treated with PBS or DT. White scale barrepresents 100 μm.

Fig4. DTR-mediated specific ablation of Mfsd2a⁺ peri-portal hepatocytes.

A, a schematic figure showing the genetic recombination of the R26-LR-tdT-DTR allele by Mfsd2a-CreER and AAV8-TBG-Dre. B, whole-mount fluorescent view of livers collected from mice treated with PBS or DT. Yellow scale bar represents 2000 μm. C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections of mice treated with PBS.White scale bar represents100 μm. E, immunostainingfor E-cad and tdTomato on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm. F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison with tdTomato+ hepatocytes (PBS group). n = 5. G, immunostaining for Tunnel and tdTomato on liver sections of mice treated with PBS or DT. White scale bar represents 100 μm.

*以上數據源自：Wang H, He L, Li Y, et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo. J Biol Chem. 2022;298(6):101965. doi:10.1016/j.jbc.2022.101965